MAB3279

antibody from Novus Biologicals

Targeting: TRAF4 CART1, MLN62, RNF83

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3279

Provider

Novus Biologicals

Product name

Rat Monoclonal TRAF-4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects endogenous human TRAF-4. In Western blots, this antibody does not cross-react with rhTRAF-1, -2, -3, -5, or -6 in Western blots.

Reactivity

Human

Host

Rat

Isotype

IgG

Vial size

250 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2.

Li J, Wang P, Xie Z, Wang S, Cen S, Li M, Liu W, Tang S, Ye G, Zheng G, Su H, Ma M, Wu X, Wu Y, Shen H
Cell death and differentiation 2019 Dec;26(12):2652-2666

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TRAF-4 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2.5 µg/mL of Human TRAF-4 Monoclonal Antibody (Catalog # MAB3279) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAF-4 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.