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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- ABIN1882055 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Topoisomerase (DNA) I (TOP1) (N-Term) antibody
- Antibody type
- Monoclonal
- Antigen
- This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
- Description
- This antibody is purified through a protein G column, followed by dialysis against PBS. Purified
- Reactivity
- Human
- Host
- Mouse
- Epitope
- N-Term
- Isotype
- IgG
- Antibody clone number
- 1291CT875-142-166
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
Submitted references Structure of the human type I DNA topoisomerase gene.
cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment.
Kunze N, Yang GC, Dölberg M, Sundarp R, Knippers R, Richter A
The Journal of biological chemistry 1991 May 25;266(15):9610-6
The Journal of biological chemistry 1991 May 25;266(15):9610-6
cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment.
D'Arpa P, Machlin PS, Ratrie H 3rd, Rothfield NF, Cleveland DW, Earnshaw WC
Proceedings of the National Academy of Sciences of the United States of America 1988 Apr;85(8):2543-7
Proceedings of the National Academy of Sciences of the United States of America 1988 Apr;85(8):2543-7
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Supportive validation
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- Experimental details
- Western blot analysis of lysates from HUVEC, Jurkat, MCF-7, PC-12 cell line (from left to right), using TOP1 Antibody (N-term) (ABIN1882055) . AM2253a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
