20705-1-AP

antibody from Proteintech Group

Targeting: TOP1

Provider product page for 20705-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [20]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 20705-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20705-1-AP, RRID:AB_10695762
Product name: TOP1 antibody
Antibody type: Polyclonal
Description: TOP1 antibody (Cat. #20705-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TOP1 protein structure - 20705-1-AP shown in red.

Submitted references Camptothecin 20(S)-sulfonyl amidine derivative inhibits gastric cancer cell proliferation by targeting topoisomerase I to trigger the DNA damage-apoptosis cascade.

Si Z, Ma Y, Cheng Z, Zhang B, Yang M, Wang C, Liu Y, Fang H, Li Y, Zhao F, Liu Y, Chen P
Bioorganic chemistry 2025 Nov;166:109171

Modulation of SUMO1-TOP1 DNA damage repair by TOPORS following ovalbumin-induced oxidative stress in macrophages.

Cai X, Xu Q, Yin T, Li X, Cheng Y, Li X, Hao C
Toxicology letters 2025 Jul;410:130-138

Oncoproteins E6 and E7 upregulate topoisomerase I to activate the cGAS-PD-L1 pathway in cervical cancer development.

Luo Y, Niu M, Liu Y, Zhang M, Deng Y, Mu D, Xu J, Hong S
Frontiers in pharmacology 2024;15:1450875

Harmine inhibits pulmonary fibrosis through regulating DNA damage repair-related genes and activation of TP53-Gadd45α pathway.

Gong Y, Wang J, Pan M, Zhao Y, Zhang H, Zhang F, Liu J, Yang J, Hu J
International immunopharmacology 2024 Sep 10;138:112542

Groenlandicine enhances cisplatin sensitivity in cisplatin-resistant osteosarcoma cells through the BAX/Bcl-2/Caspase-9/Caspase-3 pathway.

Zhao Z, Wu Q, Xu Y, Qin Y, Pan R, Meng Q, Li S
Journal of bone oncology 2024 Oct;48:100631

In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells.

Jado JC, Dow M, Carolino K, Klie A, Fonseca GJ, Ideker T, Carter H, Winzeler EA
Scientific reports 2024 Jun 18;14(1):13989

Integrated multi-omics analyses reveal Jorunnamycin A as a novel suppressor for muscle-invasive bladder cancer by targeting FASN and TOP1.

Chen R, Hao X, Chen J, Zhang C, Fan H, Lian F, Chen X, Wang C, Xia Y
Journal of translational medicine 2023 Aug 16;21(1):549

Anti-tumor effects of P-LPK-CPT, a peptide-camptothecin conjugate, in colorectal cancer.

Hou L, Hou Y, Liang Y, Chen B, Zhang X, Wang Y, Zhou K, Zhong T, Long B, Pang W, Wang L, Han X, Li L, Xu C, Gross I, Gaiddon C, Fu W, Yao H, Meng X
Communications biology 2022 Nov 14;5(1):1248

HMGB3 promotes PARP inhibitor resistance through interacting with PARP1 in ovarian cancer.

Ma H, Qi G, Han F, Lu W, Peng J, Li R, Yan S, Yuan C, Kong B
Cell death & disease 2022 Mar 24;13(3):263

Aging-associated lncRNAs are evolutionarily conserved and participate in NFκB signaling.

Cai D, Han JJ
Nature aging 2021 May;1(5):438-453

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Flor A, Wolfgeher D, Li J, Hanakahi LA, Kron SJ
Cell chemical biology 2021 Jun 17;28(6):776-787.e8

Sensitization of ovarian tumor to immune checkpoint blockade by boosting senescence-associated secretory phenotype.

Hao X, Zhao B, Zhou W, Liu H, Fukumoto T, Gabrilovich D, Zhang R
iScience 2021 Jan 22;24(1):102016

EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells.

Wu W, Dong J, Gou H, Geng R, Yang X, Chen D, Xiang B, Zhang Z, Ren S, Chen L, Liu J
Journal of cellular and molecular medicine 2021 Aug;25(16):7913-7921

Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence.

Zhao B, Liu P, Fukumoto T, Nacarelli T, Fatkhutdinov N, Wu S, Lin J, Aird KM, Tang HY, Liu Q, Speicher DW, Zhang R
Nature communications 2020 Feb 19;11(1):908

IFN-γ restores the impaired function of RNase L and induces mitochondria-mediated apoptosis in lung cancer.

Yin H, Jiang Z, Wang S, Zhang P
Cell death & disease 2019 Sep 9;10(9):642

Transcription modulation by CDK9 regulates inflammatory genes and RIPK3-MLKL-mediated necroptosis in periodontitis progression.

Li J, Shi J, Pan Y, Zhao Y, Yan F, Li H, Lei L
Scientific reports 2019 Nov 22;9(1):17369

Treatment mechanism of matrine in combination with irinotecan for colon cancer.

Duan L, Deng L, Wang D, Ma S, Li C, Zhao D
Oncology letters 2017 Aug;14(2):2300-2304

SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.

Wu J, Yang C, Guo C, Li X, Yang N, Zhao L, Hang H, Liu S, Chu P, Sun Z, Sun B, Lin Y, Peng J, Han G, Wang S, Tang Z
Chemico-biological interactions 2016 Jan 25;244:94-104

Anticancer effect of SZC017, a novel derivative of oleanolic acid, on human gastric cancer cells.

Gao L, Xu Z, Wang Y, Sun B, Song Z, Yang B, Liu X, Lin Y, Peng J, Han G, Wang S, Tang Z
Oncology reports 2016 Feb;35(2):1101-8

Genetic variants in microRNAs predict non-small cell lung cancer prognosis in Chinese female population in a prospective cohort study.

Lingzi X, Zhihua Y, Xuelian L, Yangwu R, Haibo Z, Yuxia Z, Baosen Z
Oncotarget 2016 Dec 13;7(50):83101-83114

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: L02 cells were subjected to SDS PAGE followed by western blot with 20705-1-AP(TOP1 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The TOP1 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human TOP1. This antibody recognizes human,mouse,rat antigen. The TOP1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 20705-1-AP.pdf

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