LS-B11789

antibody from LSBio

Targeting: TOP1

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B11789

Provider

LSBio

Product name

IHC-plus™ TOP1 / Topoisomerase I Antibody (aa715-765) LS-B11789

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human and Mouse TOP1 by Western Blot. Samples: Whole cell lysate from HeLa (50 ug), 293T (50 ug), Jurkat (50 ug), NIH3T3 (50 ug), and TCKM (50 ug) cells. Antibodies: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of mouse TOP1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human TOP1 by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from HeLa and (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)