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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM00154PU-N - Provider product page
- Provider
- OriGene
- Product name
- Topoisomerase I (TOP1) mouse monoclonal antibody, clone 23B11, Purified
- Antibody type
- Monoclonal
- Description
- Topoisomerase I (TOP1) mouse monoclonal antibody, clone 23B11, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TOP1
- Isotype
- IgG
- Antibody clone number
- 23B11
- Vial size
- 100 µg
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Supportive validation
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- OriGene (provider)
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- Experimental details
- Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa. Lane 2: HepG2. Lane 3: HEK-293. Lane 4: SH-SY5Y. Lane 5: MDCK. Lane 6: PC12. Lane 7: CMT-93. Lane 8: Neuro 2A. Lane 9: 3T3.
- Validation comment
- WB
