A00434-1

antibody from Boster Biological Technology

Targeting: TOP1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

A00434-1

Provider

Boster Biological Technology

Product name

Anti-Topoisomerase I/TOP1 Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for TOPOISOMERASE I/TOP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, Direct ELISA. Reactive species: Human;Mouse;Rat. TOPOISOMERASE I/TOP1 information: Subcellular Localization: Nucleus, nucleolus; Tissue Specificity: Endothelial cells.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Topoisomerase I using anti-Topoisomerase I antibody (A00434-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates,Lane 2: human COLO-320 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A431 whole cell lysates,Lane 5: human SK-OV-3 whole cell lysates,Lane 6: rat testis tissue lysates,Lane 7: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Topoisomerase I antigen affinity purified polyclonal antibody (Catalog # A00434-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Topoisomerase I at approximately 100KD. The expected band size for Topoisomerase I is at 91KD.

Additional image