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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
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- Product number
- H00007150-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007150-M01, RRID:AB_2207678
- Product name
- Mouse Monoclonal Topoisomerase I Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TOP1 - topoisomerase (DNA) I
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references AID-induced decrease in topoisomerase 1 induces DNA structural alteration and DNA cleavage for class switch recombination.
COP9 signalosome interacts ATP-dependently with p97/valosin-containing protein (VCP) and controls the ubiquitination status of proteins bound to p97/VCP.
Kobayashi M, Aida M, Nagaoka H, Begum NA, Kitawaki Y, Nakata M, Stanlie A, Doi T, Kato L, Okazaki IM, Shinkura R, Muramatsu M, Kinoshita K, Honjo T
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 29;106(52):22375-80
Proceedings of the National Academy of Sciences of the United States of America 2009 Dec 29;106(52):22375-80
COP9 signalosome interacts ATP-dependently with p97/valosin-containing protein (VCP) and controls the ubiquitination status of proteins bound to p97/VCP.
Cayli S, Klug J, Chapiro J, Fröhlich S, Krasteva G, Orel L, Meinhardt A
The Journal of biological chemistry 2009 Dec 11;284(50):34944-53
The Journal of biological chemistry 2009 Dec 11;284(50):34944-53
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in HeLa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topoisomerase I Antibody (1A1) [H00007150-M01] - TOP1 monoclonal antibody (M01), clone 1A1. Analysis of TOP1 expression in JAR.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Topoisomerase I Antibody (1A1) [H00007150-M01] - Detection limit for recombinant GST tagged TOP1 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Topoisomerase I Antibody (1A1) [H00007150-M01] - Analysis of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma. Antibody concentration 3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Topoisomerase I Antibody (1A1) [H00007150-M01] - Analysis of monoclonal antibody to TOP1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1~10 ug/ml.
