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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Other assay [2]
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Validation data
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- Product number
- MA5-38375 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TOP1 Recombinant Rabbit Monoclonal Antibody (6D8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 6D8
- Vial size
- 100 µL
- Concentration
- 0.33 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Topoisomerase I in HepG2 cells using TOP1 Monoclonal Antibody (Product # MA5-38375) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1 µg/1x10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1:200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1 µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating TOP1 in K562 whole cell lysate. Lane 1: Rabbit control IgG K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: TOP1 Monoclonal Antibody (Product # MA5-38375) (2 µg) + K562 whole cell lysate (500 µg). Lane 3: K562 whole cell lysate (10 µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitating TOP1 in K562 whole cell lysate. Lane 1: Rabbit control IgG K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1:2,000). Lane 2: TOP1 Monoclonal Antibody (Product # MA5-38375) (2 µg) + K562 whole cell lysate (500 µg). Lane 3: K562 whole cell lysate (10 µg).
