Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-30482 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-30482, RRID:AB_2207675
- Product name
- Rabbit Polyclonal Topoisomerase I Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate from HeLa (50ug), 293T (50ug), Jurkat (50ug), NIH3T3 (50ug), and TCKM (50ug) cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibody used at 0.04 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topoisomerase I Antibody [NBP1-30482] - Detection of Human TOP1 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topoisomerase I Antibody [NBP1-30482] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOP1 antibody used for WB at 0.1 ug/ml. Detection: chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Topoisomerase I Antibody [NBP1-30482] - Detection of human TOP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOP1 antibody NBP1-30482 (lot NBP1-30482-3) used for IP at 6 ug per reaction. TOP1 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-30482-2). For blotting immunoprecipitated TOP1, NBP1-30482 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.