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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab131976 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Topoisomerase I Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Topoisomerase I Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on.Application:WB: 1/500 Ð 1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein FunctionThe reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 2-15- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 0,2 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Topoisomerase I Antibody (Ab131976) - Western Blot All lanes: Recombinant Topoisomerase I Antibody (Ab131976) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 91 kDa Observed band size: 95 kDa Exposure time: 1.5 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Topoisomerase I Antibody (Ab131976) - IF/ICC Immunocytochemistry analysis of Topoisomerase I (green) in HeLa using Recombinant Topoisomerase I Antibody (Ab131976) and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Topoisomerase I Antibody (Ab131976) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Topoisomerase I Antibody (Ab131976). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
