F54611

antibody from NSJ Bioreagents

Targeting: HMMR CD168, RHAMM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54611

Provider

NSJ Bioreagents

Product name

CD168 Antibody / HMMR

Antibody type

Polyclonal

Description

This highly specific CD168 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the CD168 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) K562, 2) LNCaP and 3) HL60 cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Ramos cell lysate with CD168 antibody. Expected molecular weight ~72 kDa (cell surface form) and 85-95 kDa (intracellular form).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast carcinoma tissue with CD168 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human Ramos cells with CD168 antibody; Blue=isotype control, Green= CD168 antibody.