M00553

antibody from Boster Biological Technology

Targeting: MSH6 GTBP

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00553

Provider

Boster Biological Technology

Product name

Anti-MSH6/Gtbp Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for GTBP/MSH6 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat GTBP/MSH6 information: Molecular Weight: 152786 MW; Subcellular Localization: Nucleus . Chromosome . Associates with H3K36me3 via its PWWP domain.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AEB-13

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MSH6 Antibody(M00553)MSH6 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MSH6 Antibody (M00553)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image