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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Protein array [1]
- Other assay [1]
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- Product number
- V8011 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MSH6 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific MSH6 antibody is suitable for use in ELISA/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- MSH6/3091
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human HCT116 lysate with MSH6 antibody (clone MSH6/3091). Predicted molecular weight ~160 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MSH6/3091) and Phalloidin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MSH6/3091). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using MSH6 antibody (clone MSH6/3091). These results demonstrate the foremost specificity of the MSH6/3091 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free MSH6 antibody (clone MSH6/3091) as confirmation of integrity and purity.
