66172-1-IG

antibody from Invitrogen Antibodies

Targeting: MSH6 GTBP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66172-1-IG

Provider

Invitrogen Antibodies

Product name

MSH6 Monoclonal Antibody (2E10B2)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MSRQSTLYS FFPKSPALSD ANKASARASR EGGRAAAAPG ASPSPGGDAA WSEAGPGPRP LARSASPPKA KNLNGGLRRS VAPAAPTSCD FSPGDLVWAK MEGYPWWPCL VYNHPFDGTF IREKGKSVRV HVQFFDDSPT RGWVSKRLLK PYTGSKSKEA QKGGHFYSAK PEILRAMQRA DEALNKDKIK RLELAVCDEP SEPEEEEEME VGTTYVTDKS EEDNEIESEE EVQPKTQGSR RSSRQIKKRR VISDSESDIG GSDVEFKPDT KEEGSSDEIS SGVGDSESEG LNSPVKVARK RKRMVTGNGS LKRKSSRKET PSATKQATSI SSETKNTLRA FSAPQNSESQ AHVSGGGDDS SRPTVWYHET LEWLKEEKRR DEHRRRPDHP DFDASTLYVP E (1-400 aa encoded by BC004246)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

2E10B2

Vial size

150 µL

Concentration

1.03 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66172-1-IG ( MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of MSH6 antibody (66172-1-IG, 1:1000) with si-Control and si-MSH6 transfected HEK293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66172-1-Ig (MSH6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 66172-1-IG (MSH6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66172-1-IG ( MSH6 Antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 66172-1-IG ( MSH6 Antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HEK-293 cells were intracellularly stained with 0.2 µg Anti-Human MSH6 (Product # 66172-1-IG, Clone:2E10B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MSH6 (IP:66172-1-IG, 4ug; Detection:66172-1-IG 1:600) with HEK-293 cells lysate 1800ug.