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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NB100-328 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-328, RRID:AB_10000738
- Product name
- Goat Polyclonal MSH6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references DNA mismatch repair proteins are required for efficient herpes simplex virus 1 replication.
Hereditary cancer-associated missense mutations in hMSH6 uncouple ATP hydrolysis from DNA mismatch binding.
Mohni KN, Mastrocola AS, Bai P, Weller SK, Heinen CD
Journal of virology 2011 Dec;85(23):12241-53
Journal of virology 2011 Dec;85(23):12241-53
Hereditary cancer-associated missense mutations in hMSH6 uncouple ATP hydrolysis from DNA mismatch binding.
Cyr JL, Heinen CD
The Journal of biological chemistry 2008 Nov 14;283(46):31641-8
The Journal of biological chemistry 2008 Nov 14;283(46):31641-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MSH6 Antibody [NB100-328] - Sample: Whole cell lysate (50 ug for WB; 1 mg for IP) from HeLa (A) and HCT116 3-6 (B) cells. Antibodies: Affinity purified goat anti-MSH6 used at 1 ug/ml for WB (A) and 1 ug/mg lysate for IP (B). For IP/WB (B), MSH6 was detected using a monoclonal antibody to MSH6. Detection: Chemiluminescence with 1 min exposure.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MSH6 Antibody [NB100-328] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MSH6 antibody NB100-328 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-328] - FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti-MSH6 used at a dilution of 1:500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MSH6 Antibody [NB100-328] - Detection of human MSH6 by Immunocytochemistry, using NB100-328. Sample: HCT116 3-6 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-328] - Section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-MSH6 used at a dilution of 1:500 (2ug/ml). Detection: DAB
