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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-329 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-329, RRID:AB_10001168
- Product name
- Rabbit Polyclonal MSH6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Regulation of activation-induced deaminase stability and antibody gene diversification by Hsp90.
Mismatch Repair proteins are recruited to replicating DNA through interaction with Proliferating Cell Nuclear Antigen (PCNA).
Orthwein A, Patenaude AM, Affar el B, Lamarre A, Young JC, Di Noia JM
The Journal of experimental medicine 2010 Nov 22;207(12):2751-65
The Journal of experimental medicine 2010 Nov 22;207(12):2751-65
Mismatch Repair proteins are recruited to replicating DNA through interaction with Proliferating Cell Nuclear Antigen (PCNA).
Masih PJ, Kunnev D, Melendy T
Nucleic acids research 2008 Jan;36(1):67-75
Nucleic acids research 2008 Jan;36(1):67-75
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MSH6 Antibody [NB100-329] - Whole cell lysate (30 ug) from human lymphoblast TK6 (wildtype MSH6 expression) or MT1 (TK6 derived cells with reduced expression of a mutant MSH6). Antibody used at 0.2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MSH6 Antibody [NB100-329] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH6 antibody NB100-329 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-329] - Section of human testicular seminoma.Antibody: Affinity purified rabbit anti- MSH6 used at a dilution of 1:500 (2 ug/ml). Detection: DAB
