Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6752 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6752, RRID:AB_1577530
- Product name
- MYD88 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of MYD88.
- Antigen sequence
C-IKYKAMKKEFP
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Modulation of Toll-interleukin 1 receptor mediated signaling.
Li X, Qin J
Journal of molecular medicine (Berlin, Germany) 2005 Apr;83(4):258-66
Journal of molecular medicine (Berlin, Germany) 2005 Apr;83(4):258-66
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MYD88 polyclonal antibody (Cat # PAB6752) staining (0.03 ug/mL) of human thymus lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MYD88 polyclonal antibody (Cat # PAB6752) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL).
- Validation comment
- Sandwich ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MYD88 polyclonal antibody (Cat # PAB6752) (4 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not been obtained after antigen retireval at pH6 with this batch of antibody.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)