Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 10-3012 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-MyD88 Antibody
- Antibody type
- Monoclonal
- Description
- MyD88 (Myeloid differentiation factor) is an essential adaptor molecule in all TLR (Toll-like receptor) signaling pathways except TLR3. MyD88 is composed of an N-terminal (Death Domain) and a highly conserved C-terminal TIR (Toll/interleukin-1 Receptor) domain. It is found to stimulate IL-1R/IL18R-mediated signaling. MyD88-dependent signaling is also important in the regulation of innate as well as acquired immunity, in particular, T-cell responses, to various microbial pathogens. After activation of TLRs, MyD88 is phosphorylated and subsequently recruits IRAKs (IL-1R Associated Kinases) and other downstream proteins such as TRAF6, finally resulting in activation of the NF-kappaB (nuclear factor kappa B) pathway.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant protei
n of human MyD88 (amino acids 13-22
1) was used as an immunogen for thi
s antibody.- Isotype
- IgG
- Antibody clone number
- ABM2H20
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of MyD88. Anti-MyD88 antibody (Clone: ABM2H20) was tested at 0.1 µg/ml on h Kidney lysate.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of MyD88. Anti-MyD88 antibody (Clone: ABM2H20) was tested at 1 µg/ml on (1) Raw and (2) EL-4 lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow analysis of MyD88 in Jurkat using 0.5 µg/10^6 cells of MyD88 antibody (Clone: ABM2H20). Green represents isotype control; red represents anti-MyD88 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
- Protocol
- Protocol