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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [5]
- Other assay [1]
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- Product number
- MA5-16231 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MyD88 Monoclonal Antibody (4D6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D6
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Recognition of Double-Stranded RNA and Regulation of Interferon Pathway by Toll-Like Receptor 10.
Association between toll-like receptor 8 expression and adverse clinical outcomes in patients with enterovirus-associated dilated cardiomyopathy.
Lee SM, Yip TF, Yan S, Jin DY, Wei HL, Guo RT, Peiris JSM
Frontiers in immunology 2018;9:516
Frontiers in immunology 2018;9:516
Association between toll-like receptor 8 expression and adverse clinical outcomes in patients with enterovirus-associated dilated cardiomyopathy.
Satoh M, Akatsu T, Ishikawa Y, Minami Y, Takahashi Y, Nakamura M
American heart journal 2007 Sep;154(3):581-8
American heart journal 2007 Sep;154(3):581-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MyD88 in MCF-7 cells. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 5 µg/mL. Isotype control (top) and MyD88 Antibody (bottom).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of MyD88 in MCF-7 cells. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 5 µg/mL. Isotype control (top) and MyD88 Antibody (bottom).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in MCF7 cells (blue) and a matched isotype control (orange). Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in Jurkat cells. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 0.5 µg followed by anti-mouse IgG PE conjugated secondary antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in Jurkat cells. Samples were incubated in MyD88 monoclonal (Product # MA5-16231) using a dilution of 10 µl (0.5 µg). Green represents isotype control ; red represents anti-MyD88 antibody (conjugated to Alexa Fluor® 488).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in Jurkat cells. Samples were incubated in MyD88 monoclonal antibody (Product # MA5-16231) using a dilution of 0.5 µg followed by anti-mouse IgG PE conjugated secondary antibody. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of MyD88 in Jurkat cells. Samples were incubated in MyD88 monoclonal (Product # MA5-16231) using a dilution of 10 µl (0.5 µg). Green represents isotype control ; red represents anti-MyD88 antibody (conjugated to Alexa Fluor® 488).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
