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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB100-56698 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56698, RRID:AB_838599
- Product name
- Rabbit Polyclonal MyD88 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Because of high sequence homology, this antibody is expected to detect multiple isoforms of MyD88 protein.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references MicroRNA gga-miR-10a-mediated transcriptional regulation of the immune genes in necrotic enteritis afflicted chickens.
Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway.
Unveiling Integrated Functional Pathways Leading to Enhanced Respiratory Disease Associated With Inactivated Respiratory Syncytial Viral Vaccine.
A DNA-sensing-independent role of a nuclear RNA helicase, DHX9, in stimulation of NF-κB-mediated innate immunity against DNA virus infection.
Biphalin, a Dimeric Enkephalin, Alleviates LPS-Induced Activation in Rat Primary Microglial Cultures in Opioid Receptor-Dependent and Receptor-Independent Manners.
MAP1S Protein Regulates the Phagocytosis of Bacteria and Toll-like Receptor (TLR) Signaling.
The inflammasome pyrin contributes to pertussis toxin-induced IL-1β synthesis, neutrophil intravascular crawling and autoimmune encephalomyelitis.
Toll-like receptor 4 signaling contributes to Paclitaxel-induced peripheral neuropathy.
The transcriptomic response of rat hepatic stellate cells to endotoxin: implications for hepatic inflammation and immune regulation.
Direct extracellular interaction between the early secreted antigen ESAT-6 of Mycobacterium tuberculosis and TLR2 inhibits TLR signaling in macrophages.
Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.
A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression.
p50 nuclear factor-kappaB overexpression in tumor-associated macrophages inhibits M1 inflammatory responses and antitumor resistance.
Induction of CXCL5 during inflammation in the rodent lung involves activation of alveolar epithelium.
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line.
Pham TT, Ban J, Lee K, Hong Y, Lee J, Truong AD, Lillehoj HS, Hong YH
Developmental and comparative immunology 2020 Jan;102:103472
Developmental and comparative immunology 2020 Jan;102:103472
Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway.
Ji S, Xiao J, Liu J, Tang S
Stem cells international 2019;2019:9274585
Stem cells international 2019;2019:9274585
Unveiling Integrated Functional Pathways Leading to Enhanced Respiratory Disease Associated With Inactivated Respiratory Syncytial Viral Vaccine.
Russell MS, Creskey M, Muralidharan A, Li C, Gao J, Chen W, Larocque L, Lavoie JR, Farnsworth A, Rosu-Myles M, Hashem AM, Yauk CL, Cao J, Van Domselaar G, Cyr T, Li X
Frontiers in immunology 2019;10:597
Frontiers in immunology 2019;10:597
A DNA-sensing-independent role of a nuclear RNA helicase, DHX9, in stimulation of NF-κB-mediated innate immunity against DNA virus infection.
Ng YC, Chung WC, Kang HR, Cho HJ, Park EB, Kang SJ, Song MJ
Nucleic acids research 2018 Sep 28;46(17):9011-9026
Nucleic acids research 2018 Sep 28;46(17):9011-9026
Biphalin, a Dimeric Enkephalin, Alleviates LPS-Induced Activation in Rat Primary Microglial Cultures in Opioid Receptor-Dependent and Receptor-Independent Manners.
Popiolek-Barczyk K, Piotrowska A, Makuch W, Mika J
Neural plasticity 2017;2017:3829472
Neural plasticity 2017;2017:3829472
MAP1S Protein Regulates the Phagocytosis of Bacteria and Toll-like Receptor (TLR) Signaling.
Shi M, Zhang Y, Liu L, Zhang T, Han F, Cleveland J, Wang F, McKeehan WL, Li Y, Zhang D
The Journal of biological chemistry 2016 Jan 15;291(3):1243-50
The Journal of biological chemistry 2016 Jan 15;291(3):1243-50
The inflammasome pyrin contributes to pertussis toxin-induced IL-1β synthesis, neutrophil intravascular crawling and autoimmune encephalomyelitis.
Dumas A, Amiable N, de Rivero Vaccari JP, Chae JJ, Keane RW, Lacroix S, Vallières L
PLoS pathogens 2014 May;10(5):e1004150
PLoS pathogens 2014 May;10(5):e1004150
Toll-like receptor 4 signaling contributes to Paclitaxel-induced peripheral neuropathy.
Li Y, Zhang H, Zhang H, Kosturakis AK, Jawad AB, Dougherty PM
The journal of pain : official journal of the American Pain Society 2014 Jul;15(7):712-25
The journal of pain : official journal of the American Pain Society 2014 Jul;15(7):712-25
The transcriptomic response of rat hepatic stellate cells to endotoxin: implications for hepatic inflammation and immune regulation.
Harvey SA, Dangi A, Tandon A, Gandhi CR
PloS one 2013;8(12):e82159
PloS one 2013;8(12):e82159
Direct extracellular interaction between the early secreted antigen ESAT-6 of Mycobacterium tuberculosis and TLR2 inhibits TLR signaling in macrophages.
Pathak SK, Basu S, Basu KK, Banerjee A, Pathak S, Bhattacharyya A, Kaisho T, Kundu M, Basu J
Nature immunology 2007 Jun;8(6):610-8
Nature immunology 2007 Jun;8(6):610-8
Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.
Palladino MA, Johnson TA, Gupta R, Chapman JL, Ojha P
Biology of reproduction 2007 Jun;76(6):958-64
Biology of reproduction 2007 Jun;76(6):958-64
A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression.
Macagno A, Molteni M, Rinaldi A, Bertoni F, Lanzavecchia A, Rossetti C, Sallusto F
The Journal of experimental medicine 2006 Jun 12;203(6):1481-92
The Journal of experimental medicine 2006 Jun 12;203(6):1481-92
p50 nuclear factor-kappaB overexpression in tumor-associated macrophages inhibits M1 inflammatory responses and antitumor resistance.
Saccani A, Schioppa T, Porta C, Biswas SK, Nebuloni M, Vago L, Bottazzi B, Colombo MP, Mantovani A, Sica A
Cancer research 2006 Dec 1;66(23):11432-40
Cancer research 2006 Dec 1;66(23):11432-40
Induction of CXCL5 during inflammation in the rodent lung involves activation of alveolar epithelium.
Jeyaseelan S, Manzer R, Young SK, Yamamoto M, Akira S, Mason RJ, Worthen GS
American journal of respiratory cell and molecular biology 2005 Jun;32(6):531-9
American journal of respiratory cell and molecular biology 2005 Jun;32(6):531-9
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line.
Srivastava MD, Srivastava BI
Leukemia research 2005 Jul;29(7):813-20
Leukemia research 2005 Jul;29(7):813-20
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyD88 Antibody [NB100-56698] - Analysis of MyD88 in human spleen cell lysate using 0.5 ug/ml of NB100-56698.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyD88 Antibody [NB100-56698] - The ability of PTX to induce the IL-1 Beta IL-6 cascade depends on the integrity of its multimeric structure and enzymatic activity. Western blots revealing the presence of mature IL-1 Beta, cleaved Casp1, TLR4 and Myd88 in peritoneal fluid or peritoneal cell lysates from mice killed 6 h after injection of PBS, wild-type PTX or PTXmut. Arrowheads indicate the cleaved forms. Beta-actin was used as loading control. Sample size: 8 per group. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.ppat.1004150) licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MyD88 Antibody [NB100-56698] - Tissue section of human liver using at 1:100 dilution. This antibody generated a very specific cytoplasmic staining in the hepatocytes as well as the Kupffer cells (hepatic macrophages), and the latter showed the most intense staining among all other cell types in the section.
