Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX100231 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100231, RRID:AB_1240689
- Product name
- DCTN1 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: JurKat 5% SDS PAGE GTX100231 diluted at 1:3000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DCTN1(GTX100231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using dynactin 1(GTX100231) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DCTN1 antibody [C3], C-term detects DCTN1 protein at cytoplasm in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:500.