NB100-1110

antibody from Novus Biologicals

Targeting: DCTN1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

NB100-1110

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1110, RRID:AB_2279060

Product name

Goat Polyclonal Dynactin Subunit 1/DCTN1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This antibody is expected to recognise all reported isoforms (NP_004073.2

Reactivity

Human, Mouse, Rabbit

Host

Goat

Isotype

IgG

Vial size

0.1 mg

Concentration

0.5 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

The hnRNP RALY regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization.

Gasperini L, Rossi A, Cornella N, Peroni D, Zuccotti P, Potrich V, Quattrone A, Macchi P
Molecular biology of the cell 2018 Dec 15;29(26):3067-3081

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Temporal changes in expression of connexin 43 after load-induced hypertrophy in vitro.

Bupha-Intr T, Haizlip KM, Janssen PM
American journal of physiology. Heart and circulatory physiology 2009 Mar;296(3):H806-14

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Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization.

Holzbaur EL, Tokito MK
Genomics 1996 Feb 1;31(3):398-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (1ug/ml) of Human Testis lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (2.5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.