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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF6657 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Dynactin Subunit 1/DCTN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Dynactin Subunit 1/DCTN1 in direct ELISAs and human, mouse and rat Dynactin Subunit 1/DCTN1 in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q14203- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Dynactin Subunit 1/DCTN1 by Western Blot. Western blot shows lysates of human brain tissue, human testis tissue, mouse brain tissue, and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human Dynactin Subunit 1/DCTN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6657) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Dynactin Subunit 1/DCTN1 at approximately 150 and 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DCTN1 in Human Brain. DCTN1 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Sheep Anti-Human/Mouse/Rat Dynactin Subunit 1/DCTN1 Antigen Affinity Purified Polyclonal Antibody (Catalog # AF6657) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
