sc-11369

antibody from Santa Cruz Biotechnology

Targeting: IPO5 IMB3, KPNB3, MGC2068, Pse1, RANBP5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-11369

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-11369, RRID:AB_2264985

Product name

Anti-IPO5

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Sequential establishment of marks on soluble histones H3 and H4.

Alvarez F, Muñoz F, Schilcher P, Imhof A, Almouzni G, Loyola A
The Journal of biological chemistry 2011 May 20;286(20):17714-21

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The canine papillomavirus e5 protein signals from the endoplasmic reticulum.

Condjella R, Liu X, Suprynowicz F, Yuan H, Sudarshan S, Dai Y, Schlegel R
Journal of virology 2009 Dec;83(24):12833-41

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 14

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.