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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-96023 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IPO5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AAAAAEQQQF YLLLGNLLSP DNVVRKQAEE TYENIPGQSK ITFLLQAIRN TTAAEEARQM AAVLLRRLLS SAFDEVYPAL PSDVQTAIKS ELLMIIQMET QSSMRKKVCD IAAELARNLI DEDGNNQWPE GLKFLFDSVS SQNVGLREAA LHIFWNFPGI FGNQQQHYLD VIKRMLVQCM QDQEHPSIRT LSARATAAFI LANEHNVALF KHFADLLPGF LQAVNDSCYQ NDDSVLKSLV EIADTVPKYL RPHLEATLQL SLKLCGDTSL NNMQRQLALE V; Positive Samples: SW620, HeLa, 293T, HepG2, Mouse testis, Mouse brain; Cellular Location: Cytoplasm, Nucleus, nucleolus Excitation: 405 nm; Emission: 645 nm; Laser: Blue Laser
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 3.31 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IPO5 in mouse testis. Samples were incubated with IPO5 Polyclonal antibody (Product # PA5-96023) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IPO5 in rat testis. Samples were incubated with IPO5 Polyclonal antibody (Product # PA5-96023) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IPO5 was performed in U2OS cells using IPO5 Polyclonal Antibody (Product # PA5-96023).
