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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA324579 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal SMAD9 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal SMAD9 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SMAD9
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.45 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SMAD9 Antibody (Center) (Cat. #TA324579) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SMAD9 antibody detected the SMAD9 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of MCF-7 cell stained with SMAD9 Antibody (Center)(Cat#TA324579). MCF-7 cells were incubated with SMAD9 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.
- Validation comment
- IF
