sc-11412

NR3C2 antibody from Santa Cruz Biotechnology

MLR, MR

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-11412

Antibody Data

Product number

sc-11412

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-11412, RRID:AB_2155949

Product name

Anti-NR3C2

Provider product page

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Prenatal synthetic glucocorticoid exposure alters hypothalamic-pituitary-adrenal regulation and pregnancy outcomes in mature female guinea pigs.
Dunn E, Kapoor A, Leen J, Matthews SG
The Journal of physiology 2010 Mar 1;588(Pt 5):887-99

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Aldosterone activates endothelial exocytosis.
Jeong Y, Chaupin DF, Matsushita K, Yamakuchi M, Cameron SJ, Morrell CN, Lowenstein CJ
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 10;106(10):3782-7

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Hepatocyte nuclear factor-1alpha regulates glucocorticoid receptor expression to control postnatal body growth.
Lin WY, Hu YJ, Lee YH
American journal of physiology. Gastrointestinal and liver physiology 2008 Sep;295(3):G542-51

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Molecular regulation of the hypothalamic-pituitary-adrenal axis in adult male guinea pigs after prenatal stress at different stages of gestation.
Kapoor A, Leen J, Matthews SG
The Journal of physiology 2008 Sep 1;586(Pt 17):4317-26

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Effects of repeated prenatal glucocorticoid exposure on long-term potentiation in the juvenile guinea-pig hippocampus.
Setiawan E, Jackson MF, MacDonald JF, Matthews SG
The Journal of physiology 2007 Jun 15;581(Pt 3):1033-42

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 213, 118, 83, 50, 33, 26, 18

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 108

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human kidney shows distinct granular cytoplasmic positivity in cells in tubules.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.