GTX22774
antibody from GeneTex
Targeting: NR3C2
MLR, MR
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- GTX22774 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22774, RRID:AB_384844
- Product name
- Mineralocorticoid Receptor antibody [H10E4C9F]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Rabbit, Sheep
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of Mineralocorticoid Receptor.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of Mineralocorticoid Receptor using GTX22774
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Mineralocorticoid Receptor in HEK293 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Mineralocorticoid Receptor antibody [H10E4C9F] at a dilution of 1:20-1:200 over night at 4 ¢XC, washed with PBS and incubated with a proper secondary antibody. Images were taken at 60X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on cancer biopsies of deparaffinized human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with or without Mineralocorticoid Receptor antibody [H10E4C9F] overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.