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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- R31912 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MR Antibody / Mineralocorticoid Receptor
- Antibody type
- Polyclonal
- Description
- This highly specific MR antibody is suitable for use in Western blot/Immunofluorescence applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the MR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat kidney, 2) mouse kidney, 3) human HeLa and 4) human A431 lysate with MR antibody. Expected molecular weight ~107/108/94 kDa (isoforms 1/3/4).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) huma HeLa, 2) human 293T, 3) human LNCaP, 4) human MCF7, 5) rat NRK and 6) mouse NIH 3T3 cell lysate with MR antibody. Expected molecular weight ~107/108/94 kDa (isoforms 1/3/4).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with MR antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
