21854-1-AP

antibody from Proteintech Group

Targeting: NR3C2 MLR, MR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21854-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21854-1-AP, RRID:AB_10858785

Product name

NR3C2 antibody

Antibody type

Polyclonal

Description

NR3C2 antibody (Cat. #21854-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Astragalus polysaccharide hinders cervical cancer immune escape by targeting NR3C2 and activating SLC40A1.

Liu W, Zhang L, Wang Y, Jiang X, Tan J, Zhang Y, Fu Y
Biochimica et biophysica acta. Molecular basis of disease 2026 Mar;1872(3):168123

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Esaxerenone attenuates high salt-induced hypertension and renal damage via CD8(+)T cell- associated NCC activation in aldosterone treated rats.

Xu C, Zhai N, Wang H, Fang F, An J, Liu F, Zhong Y, Wang Z, Wang X
The Journal of steroid biochemistry and molecular biology 2026 Jan;255:106884

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Acute antilipolytic effects of elevated levels of corticosterone in differentiated murine white adipocytes.

Mir N, Lyons SA, Guo Z, Mirza SK, Tan W, Peckett AJ, Beaudry JL
American journal of physiology. Cell physiology 2025 Sep 1;329(3):C972-C985

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Tubeimoside I inhibits the growth of triple-negative breast cancer via the NR3 C2/PI3 K/AKT signaling pathway.

Zhang Q, Cheng W, Li Y, Zhang Y, Yu Y, Wei S, Yang Y, Zhang W, Liu C, Wu Q, Sun C
Naunyn-Schmiedeberg's archives of pharmacology 2025 Nov;398(11):15377-15390

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Aldosterone-Induced Renal Lymphangiogenesis and Endothelial-To-Mesenchymal Transformation to Promote Renal Interstitial Fibrosis Through the MR/TGF-β1 Pathway in Mice.

Fan L, Liu Z, Chang Y, Xiong Y, Yang F, Gao X, Chang J, Shimosawa T, Xu Q, Qiang P
Frontiers in bioscience (Landmark edition) 2025 Nov 27;30(11):45591

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Aldosterone induces renal lymphangiogenesis through macrophage-lymphatic endothelial cell transformation and Inhibition by esaxerenone.

Guo H, Liu Z, Lv R, Zhang B, Qiang P, Wang X, Chang Y, Yang F, Shimosawa T, Xu Q, Xiong Y
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2025 May 24;74(1):85

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Proliferation of renal macrophage via MR/CSF1 pathway induced with aldosterone and inhibited by esaxerenone.

Liu Z, Lv R, Guo H, Zhang B, Wang X, Qiang P, Xiong Y, Chang Y, Peng Y, Hao J, Wang X, Shimosawa T, Xu Q, Yang F
International immunopharmacology 2025 Mar 6;149:114208

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Corticosteroid-regulated gene transcription in SH-SY5Y-derived neurons: Insights into the mineralocorticoid and glucocorticoid receptor-mediated response.

Lugenbühl JF, Snijders C, Pernia CD, Estruch MS, Kenis G, Daskalakis NP
Journal of neuroendocrinology 2025 Jul;37(7):e70021

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Xiaoyu Xiezhuo Decoction Ameliorates Tubulointerstitial Fibrosis by Modulating Gut-Kidney Axis and Aldosterone Signaling Pathway in UUO Rats.

Sun K, Huang F, Xie W, Hu W, Zhu M, Fan Z, Lu K
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2025 Jul 31;39(14):e70861

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Fibroblast to macrophage-like cell transition in renal inflammatory injury through the MR/CSF1 pathway induced by aldosterone.

Lv R, Liu Z, Guo H, Zhang B, Wang X, Peng Y, Chang Y, Yang F, Xiong Y, Hao J, Gao X, Wang X, Xu Q, Shimosawa T, Qiang P
Life sciences 2025 Jul 1;372:123627

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Esaxerenone inhibits renal macrophage proliferation through MR/TGF-β1 pathway in db/db mice.

Yang F, Liu Z, Li Y, Lv R, Zhao Y, Yang F, Shimosawa T, Tan J, Xu Q, Qiang P
Life sciences 2025 Dec 1;382:124008

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Mineralocorticoid receptor antagonism improves corneal integrity in a rat model of limbal stem cell deficiency.

Rodrigues-Braz D, Bonnet C, Zhu L, Yesilirmak N, Gélizé E, Jonet L, Jaisser F, Bourges JL, Behar-Cohen F, Zhao M
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2025 Apr;185:117979

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Aldosterone-Induced Transformation of Vascular Smooth Muscle Cells into Macrophage-like Cells Participates in Inflammatory Vascular Lesions.

Zhang B, Liu Z, Chang Y, Lv R, Guo H, Qiang P, Shimosawa T, Xu Q, Yang F
International journal of molecular sciences 2025 Apr 3;26(7)

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Effect and underlying mechanism of Huangjing Qianshi decoction in pre-diabetes mouse model.

Cai J, Zhu Y, Deng G, Ouyang L, Xiao W, Zhou F, Han Y, Yuan F, Huang L, Li X
BMC complementary medicine and therapies 2025 Apr 23;25(1):151

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Mineralocorticoid receptor expression and the effects of the mineralocorticoid receptor antagonist spironolactone in a murine model of graft-versus-host disease.

Sato S, Ogawa Y, Wong CW, Le HL, Yee RW, Gombos DS, Negishi K, Hirayama M
The ocular surface 2024 Oct;34:477-488

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An in Vitro triple screen model for human mineralocorticoid receptor activity.

Liu H, Konzen S, Coy A, Rege J, Gomez-Sanchez CE, Rainey WE, Turcu AF
The Journal of steroid biochemistry and molecular biology 2024 Oct;243:106568

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Increased adrenal steroidogenesis and suppressed corticosteroid responsiveness in critical COVID-19.

Wen TZ, Li TR, Chen XY, Chen HY, Wang S, Fu WJ, Xiao SQ, Luo J, Tang R, Ji JL, Huang JF, He ZC, Luo T, Zhao HL, Chen C, Miao JY, Niu Q, Wang Y, Bian XW, Yao XH
Metabolism: clinical and experimental 2024 Nov;160:155980

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Eplerenone reduces lymphangiogenesis in the contralateral kidneys of UUO rats.

Hao J, Qiang P, Fan L, Xiong Y, Chang Y, Yang F, Wang X, Shimosawa T, Mu S, Xu Q
Scientific reports 2024 May 1;14(1):9976

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Esaxerenone Attenuates Aldosterone-Induced Mitochondrial Damage-Mediated Pyroptosis in Mouse Aorta and Rat Vascular Smooth Muscle Cells.

Xian Y, Wang X, Chang Y, Qiang P, Han Y, Hao J, Gao X, Shimosawa T, Xu Q, Yang F
Life (Basel, Switzerland) 2024 Jul 31;14(8)

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Propofol-induced LINC01133 inhibits the progression of colorectal cancer via miR-186-5p/NR3C2 axis.

Yao Y, Zhang F, Liu F, Xia D
Environmental toxicology 2024 Apr;39(4):2265-2284

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Disorganized adrenocortical zonational structure in COVID-19 patients: Implications of critical illness duration.

Wen TZ, Fu WJ, Xiao SQ, Wang S, Li TR, Chen XY, Chen HY, Luo J, Bian XW, Yao XH
Pathology, research and practice 2024 Apr;256:155251

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Prognostic model construction and target identification of Si-Wu-Tang against breast cancer.

Zhang Z, Zhang Z, Song J, Wu W, Chen Y, Li J, Wang Y, Zhao P
Heliyon 2023 Oct;9(10):e20709

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Validation of prognostic signature and exploring the immune-related mechanisms for NR3C2 in clear cell renal cell carcinoma.

Chen D, Yin Z, Chen Y, Bai Y, You B, Sun Y, Wu Y
Translational cancer research 2023 Oct 31;12(10):2518-2532

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The mineralocorticoid receptor modulates timing and location of genomic binding by glucocorticoid receptor in response to synthetic glucocorticoids in keratinocytes.

Carceller-Zazo E, Sevilla LM, Pons-Alonso O, Chiner-Oms Á, Amazit L, An Vu T, Vitellius G, Viengchareun S, Comas I, Jaszczyszyn Y, Abella M, Alegre-Martí A, Estébanez-Perpiñá E, Lombès M, Pérez P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2023 Jan;37(1):e22709

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NR3C2 mediates oxidised low-density lipoprotein-induced human coronary endothelial cells dysfunction via modulation of NLRP3 inflammasome activation.

Chen X, Li W, Chang C
Autoimmunity 2023 Dec;56(1):2189135

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MicroRNA-155-5p Targets NR3C2 to Promote Malignant Progression of Clear Cell Renal Cell Carcinoma.

Yan C, Wang P, Zhao C, Yin G, Meng X, Li L, Cai S, Meng B
Kidney & blood pressure research 2022;47(5):354-362

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Eplerenone Prevents Cardiac Fibrosis by Inhibiting Angiogenesis in Unilateral Urinary Obstruction Rats.

Chang Y, Ben Y, Li H, Xiong Y, Chen G, Hao J, Ma X, Gao X, Qiang P, Shimosawa T, Wang X, Yang F, Xu Q
Journal of the renin-angiotensin-aldosterone system : JRAAS 2022;2022:1283729

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Esaxerenone inhibits the macrophage-to-myofibroblast transition through mineralocorticoid receptor/TGF-β1 pathway in mice induced with aldosterone.

Qiang P, Hao J, Yang F, Han Y, Chang Y, Xian Y, Xiong Y, Gao X, Liang L, Shimosawa T, Xu Q
Frontiers in immunology 2022;13:948658

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High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.

Yuan Y, Miao Y, Ren T, Huang F, Qian L, Chen X, Zuo Y, Zhang HG, He J, Qiao C, Du Q, Wu Q, Zhang W, Zhu C, Xu Y, Wu D, Shi W, Jiang J, Xu G, Zheng H
EMBO reports 2022 Jan 5;23(1):e53466

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Melatonin prevents experimental central serous chorioretinopathy in rats.

Yu S, Cui K, Wu P, Wu B, Lu X, Huang R, Tang X, Lin J, Yang B, Zhao J, He Q, Liang X, Xu Y
Journal of pineal research 2022 Aug;73(1):e12802

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Huoxue Jiedu Huayu Formula Alleviates Cell Pyroptosis in Contralateral Kidneys of 6-Month-Old UUO Rats through the NLRP3/Caspase-1/IL-1β Pathway.

Ma X, Qiang P, Chen G, Wang Z, Wang X, Xu Q
Evidence-based complementary and alternative medicine : eCAM 2021;2021:5533911

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UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.

Yang F, Chang Y, Zhang C, Xiong Y, Wang X, Ma X, Wang Z, Li H, Shimosawa T, Pei L, Xu Q
International immunopharmacology 2021 Apr;93:107396

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Age‑related changes in mineralocorticoid receptors in rat hearts.

Hu D, Dong R, Zhang Y, Yang Y, Chen Z, Tang Y, Fu M, Xu X, Tu L
Molecular medicine reports 2020 Sep;22(3):1859-1867

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Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Hypertension by Modulating the Hsa-miR-124-3p/Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2) and Hsa-miR-135a-5p/NR3C2 Axis.

Luo L, Wang Y, Hu P, Wu J
Medical science monitor : international medical journal of experimental and clinical research 2020 Mar 29;26:e920478

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Aldosterone from endometrial glands is benefit for human decidualization.

Li SY, Song Z, Yan YP, Li B, Song MJ, Liu YF, Yang ZS, Li MY, Liu AX, Quan S, Yang ZM
Cell death & disease 2020 Aug 13;11(8):679

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Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Wang D, Xu B, Wang J, Wang H, Guo J, Ji H, Li S, Wu R, Yang H, Lian S
Brain research 2019 Nov 1;1722:146354

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Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro.

Yang Y, Chen S, Tao L, Gan S, Luo H, Xu Y, Shen X
Medical science monitor : international medical journal of experimental and clinical research 2019 Jul 20;25:5375-5388

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Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Lian S, Xu B, Wang D, Wang L, Li W, Yao R, Ji H, Wang J, Guo J, Li S, Yang H
Behavioural brain research 2019 Feb 1;359:304-311

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Identification of mineralocorticoid receptor target genes in the mouse hippocampus.

van Weert LTCM, Buurstede JC, Sips HCM, Vettorazzi S, Mol IM, Hartmann J, Prekovic S, Zwart W, Schmidt MV, Roozendaal B, Tuckermann JP, Sarabdjitsingh RA, Meijer OC
Journal of neuroendocrinology 2019 Aug;31(8):e12735

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Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Lian S, Wang D, Xu B, Guo W, Wang L, Li W, Ji H, Wang J, Kong F, Zhen L, Li S, Zhang L, Guo J, Yang H
Behavioural brain research 2018 Jul 2;346:1-10

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Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

Lian S, Guo J, Wang L, Li W, Wang J, Ji H, Kong F, Xu B, Li S, Yang H
Oncotarget 2017 Dec 29;8(70):115304-115314

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 21854-1-AP(NR3C2 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NR3C2 antibody 21854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 21854-1-AP(NR3C2 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human kidney using 21854-1-AP(NR3C2 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue