MA1-620

antibody from Invitrogen Antibodies

Targeting: NR3C2 MLR, MR

Provider product page for MA1-620

Western blot

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [26]
Comments [0]
Validations
Immunocytochemistry [3]
Other assay [4]

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Product number: MA1-620 - Provider product page
Provider: Invitrogen Antibodies
Product name: NR3C2 Monoclonal Antibody (H10E4C9F)
Antibody type: Monoclonal
Antigen: Other
Description: MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit and chicken tissues. MA1-620 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate. Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells. MA1-620 cannot be used for immunoprecipitation. In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone. MA1-620 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. MA1-620 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. The antibody can also react with the steroid binding domain for aldosterone on other receptors. The MA1-620 immunogen is aldosterone-3. This antibody was produced using the anti-idiotypic method.
Reactivity: Human, Rat, Chicken/Avian, Rabbit
Host: Mouse
Isotype: IgG
Antibody clone number: H10E4C9F
Vial size: 100 µL
Concentration: 1.4 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

NR3C2 protein structure - MA1-620 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product # MA1-620) shows staining in HEK293 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product # MA1-620) at a dilution of 1:20-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product # MA1-620) shows staining in HeLa cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product # MA1-620) at a dilution of 1:20-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product # MA1-620) shows staining in HepG2 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product # MA1-620) at a dilution of 1:20-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.