R31616

antibody from NSJ Bioreagents

Targeting: GPX1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31616

Provider

NSJ Bioreagents

Product name

GPX1 Antibody / Glutathione Peroxidase 1

Antibody type

Polyclonal

Description

This highly specific GPX1 antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the GPX1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of GPX1 antibody and Lane 1: rat kidney; 2: mouse kidney; 3: (r) spleen; 4: human HeLa; 5: (h) Jurkat. Predicted/observed size ~21KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of GPX1 antibody and recombinant human protein (0.5ng)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing 1) human ThP-1, 2) rat lung, 3) rat liver, 4) mouse lung and 5) mouse liver lysate with GPX1 antibody. Predicted molecular weight ~22 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GPX1 antibody testing of human intestine cancer tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GPX1 antibody testing of mouse pancreas tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: GPX1 antibody testing of rat pancreas tissue

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colorectal adenocarcinoma tissue with GPX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat lung tissue with GPX1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.