PA5-57575

antibody from Invitrogen Antibodies

Targeting: TOMM40 C19orf1, D19S1177E, PER-EC1, PEREC1, TOM40

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-57575

Provider

Invitrogen Antibodies

Product name

TOMM40 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FTLPPLGGSL GAGTSTSRSS ERTPGAATAS ASGAAEDGAC GCLPNPGTFE ECHRKCKELF PIQM Highest antigen sequence identity to the following orthologs: Mouse - 80%, Rat - 73%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Callyspongiolide kills cells by inducing mitochondrial dysfunction via cellular iron depletion.

Ha J, Park SB
Communications biology 2021 Sep 23;4(1):1123

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of TOMM40 in human cell line HEK 293 shows positivity in mitochondria. Samples were probed using a TOMM40 Polyclonal Antibody (Product # PA5-57575).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TOMM40 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TOMM40 Polyclonal Antibody (Product # PA5-57575) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.