PA5-53764

antibody from Invitrogen Antibodies

Targeting: SMARCB1 BAF47, hSNFS, Ini1, PPP1R144, RDT, Sfh1p, SNF5, SNF5L1, Snr1

Antibody data

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Validation data

Product number: PA5-53764 - Provider product page
Provider: Invitrogen Antibodies
Product name: SMARCB1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: SVTLLKASEV EEILDGNDEK YKAVSISTEP PTYLREQKAK RNSQWVPTLP NSSHHLDAVP CSTTINRNRM GRDKKRTFPL CFDDHDPAVI HENASQPE Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

SMARCB1 protein structure - PA5-53764 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SMARCB1 in human cell line HEK 293 using a SMARCB1 Polyclonal Antibody (Product # PA5-53764).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SMARCB1 using SMARCB1 Polyclonal Antibody (Product # PA5-53764) (A) shows similar pattern to an independent SMARCB1 Polyclonal Antibody (B).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of SMARCB1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a SMARCB1 Polyclonal Antibody (Product # PA5-53764).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of SMARCB1 was achieved by transfecting HeLa cells with SMARCB1 specific siRNAs (Silencer® select Product # s529255, s13142). Western blot analysis (Fig. a) was performed using modified whole cell extracts from the HeLa knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMACRB1 Rabbit Polyclonal Antibody (Product # PA5-53764, 0.4 µg/mL) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMARCB1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-SMARCB1 Polyclonal Antibody (Product # PA5-53764) and a ~44 kDa band was observed across the panel tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of A549 (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), HEK-293 (Lane 5), MCF-7 (Lane 6), U-2 OS (Lane 7), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 8) and Rat Spleen (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of SMARCB1 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a SMARCB1 Polyclonal Antibody (Product # PA5-53764).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent staining of SMARCB1 in human cell line A-431 using a SMARCB1 Polyclonal Antibody (Product # PA5-53764) shows localization to nucleoplasm and vesicles.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of SMARCB1 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMARCB1 Rabbit Polyclonal Antibody (Product # PA5-53764) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells, showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.