PA5-53932
antibody from Invitrogen Antibodies
Targeting: SMARCB1
BAF47, hSNFS, Ini1, PPP1R144, RDT, Sfh1p, SNF5, SNF5L1, Snr1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-53932 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: EKENSPEKFA LKLCSELGLG GEFVTTIAYS IRGQLSWHQK TYAFSENPLP TVEIAIRNTG DADQWCPLLE TLTDAEMEKK IRDQDRNTRR MRRLANTA Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCB1 in mouse cell line NIH-3T3 and rat cell line NBT-II using a SMARCB1 Polyclonal Antibody (Product # PA5-53932).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMARCB1 using SMARCB1 Polyclonal Antibody (Product # PA5-53932) (A) shows similar pattern to an independent SMARCB1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SMARCB1 was achieved by transfecting HeLa cells with SMARCB1 specific siRNAs (Silencer® select Product # s529255, s13142). Western blot analysis (Fig. a) was performed using modified whole cell extracts from the HeLa knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SMACRB1 Rabbit Polyclonal Antibody (Product # PA5-53932, 0.4 µg/mL) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SMARCB1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SMARCB1 Polyclonal Antibody (Product # PA5-53932) and a ~44 kDa band was observed across the panel tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of A549 (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), HEK-293 (Lane 5), MCF-7 (Lane 6), U-2 OS (Lane 7), tissue extracts (30 µg lysate) of Mouse Spleen (Lane 8) and Rat Spleen (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SMARCB1 in human cell line A-431 shows positivity in nucleus & nucleoli. Samples were probed using a SMARCB1 Polyclonal Antibody (Product # PA5-53932).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SMARCB1 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMARCB1 Rabbit Polyclonal Antibody (Product # PA5-53932) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green) in HeLa cells. Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image of HeLa cells, showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SMARCB1 in human tonsil using a SMARCB1 Polyclonal Antibody (Product # PA5-53932) shows nuclear positivity.