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LearnA301-087A
antibody from Invitrogen Antibodies
Targeting: SMARCB1
BAF47, hSNFS, Ini1, PPP1R144, RDT, Sfh1p, SNF5, SNF5L1, Snr1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- A301-087A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMARCB1/SNF5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (Product # A301-087A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (Product # A301-087A lot 3) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody A301-087A (lot A301-087A-2) used for IP at 6 µg per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody A301-087A-1. For blotting immunoprecipitated SMARCB1/SNF5, A301-087A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SMARCB1/SNF5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCB1/SNF5 antibody A301-087A (lot A301-087A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti SMARCB1/SNF5 (Product # A301-087A; lot 3) used at a dilution of 1:2,000 (0.5 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 (Cat. No. A301-087A Lot 2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti- SMARCB1/SNF5 (Cat. No. A301-087A Lot 2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SMARCB1/SNF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCB1/SNF5 antibody (Product # A301-087A lot 3) used for IP at 6 µg per reaction. SMARCB1/SNF5 was also immunoprecipitated by a previous lot of this antibody (A301-087A lot 2) and a second antibody against a different epitope of SMARCB1/SNF5 (BL708). For blotting immunoprecipitated SMARCB1/SNF5 (Product # A301-087A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
