Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
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Validation data
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- Product number
- H00008940-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008940-M01, RRID:AB_548758
- Product name
- Mouse Monoclonal Topo III beta Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TOP3B - topoisomerase (DNA) III beta
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B over-expressed 293 cell line, cotransfected with TOP3B Validated Chimera RNAi ( Cat # H00008940-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOP3B monoclonal antibody (M01), clone 4F11 (Cat # H00008940-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - Analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M01), clone 4F11.Lane 1: TOP3B transfected lysate(96.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Topo III beta Antibody (4F11) [H00008940-M01] - TOP3B monoclonal antibody (M01), clone 4F11 Analysis of TOP3B expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Topo III beta Antibody (4F11) [H00008940-M01] - Detection limit for recombinant GST tagged TOP3B is approximately 1ng/ml as a capture antibody.