Ab109665

antibody from Aladdin Scientific

Targeting: IHH BDA1, HHG2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab109665

Provider

Aladdin Scientific

Product name

Recombinant Ihh Antibody

Antibody type

Recombinant

Description

Rabbit anti Human Ihh Antibody, Recombinant (R03-3F2), could be used for WB, IHC and so on.ApplicationWB: 1/500-1/1000IHC: 1/50-1/100Protein FunctionIntercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 28-42


Antibody clone number

R03-3F2

Vial size

100μl,10μl,1ml,50μl

Concentration

0,3 mg/ml

Storage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Ihh Antibody (Ab109665) - Western Blot All lanes: Recombinant Ihh Antibody (Ab109665) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 41 kDa Exposure time: 10.7 s

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Ihh Antibody (Ab109665) - IHC Immunohistochemistry analysis of paraffin-embedded mouse colon using Recombinant Ihh Antibody (Ab109665). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.