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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF578 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IGFBP-5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IGFBP-5 in ELISAs and Western blots. In sandwich ELISAs, less than 3% cross-reactivity with recombinant human IGFBP-5 is observed, and less than 0.3% cross-reactivity with recombinant mouse (rm) IGF-I, rmIGF-II, rmIGFBP-1, rmIGFBP-2, rmIGFBP-3, and rmIGFBP-6 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The putative role of insulin-like growth factor (IGF)-binding protein 5 independent of IGF in the maintenance of pulpal homeostasis in mice.
Molecular determinants of nephron vascular specialization in the kidney.
Pappa2 deletion in mice affects male but not female fertility.
Saito K, Ohshima H
Regenerative therapy 2019 Dec;11:217-224
Regenerative therapy 2019 Dec;11:217-224
Molecular determinants of nephron vascular specialization in the kidney.
Barry DM, McMillan EA, Kunar B, Lis R, Zhang T, Lu T, Daniel E, Yokoyama M, Gomez-Salinero JM, Sureshbabu A, Cleaver O, Di Lorenzo A, Choi ME, Xiang J, Redmond D, Rabbany SY, Muthukumar T, Rafii S
Nature communications 2019 Dec 13;10(1):5705
Nature communications 2019 Dec 13;10(1):5705
Pappa2 deletion in mice affects male but not female fertility.
Christians JK, King AY, Rogowska MD, Hessels SM
Reproductive biology and endocrinology : RB&E 2015 Sep 29;13:109
Reproductive biology and endocrinology : RB&E 2015 Sep 29;13:109
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IGFBP-5 in Mouse Ovary. IGFBP-5 was detected in perfusion fixed frozen sections of mouse ovary (follicle with oocyte) using 5 µg/mL Goat Anti-Mouse IGFBP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF578) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
