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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF875 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IGFBP-5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IGFBP-5 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse IGFBP-5 is observed and less than 5% cross-reactivity with recombinant human (rh) IGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4, rhIGFBP-6, and rhIGFBP-7 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Netazepide Inhibits Expression of Pappalysin 2 in Type 1 Gastric Neuroendocrine Tumors.
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
Lloyd KA, Parsons BN, Burkitt MD, Moore AR, Papoutsopoulou S, Boyce M, Duckworth CA, Exarchou K, Howes N, Rainbow L, Fang Y, Oxvig C, Dodd S, Varro A, Hall N, Pritchard DM
Cellular and molecular gastroenterology and hepatology 2020;10(1):113-132
Cellular and molecular gastroenterology and hepatology 2020;10(1):113-132
IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
Crosley EJ, Dunk CE, Beristain AG, Christians JK
Reproductive biology and endocrinology : RB&E 2014 Dec 4;12:123
Reproductive biology and endocrinology : RB&E 2014 Dec 4;12:123
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Nishizawa H, Pryor-Koishi K, Suzuki M, Kato T, Kogo H, Sekiya T, Kurahashi H, Udagawa Y
Molecular human reproduction 2008 Oct;14(10):595-602
Molecular human reproduction 2008 Oct;14(10):595-602
The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
Gastroenterology 2006 May;130(6):1754-63
Gastroenterology 2006 May;130(6):1754-63
Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
Biochemical and biophysical research communications 2005 Aug 5;333(3):1011-6
Biochemical and biophysical research communications 2005 Aug 5;333(3):1011-6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IGFBP-5 in Human Placenta. IGFBP-5 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGFBP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF875) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
