Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319458 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-TrkCT1 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-TrkCT1 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Ntrk3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.53 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-TrkCT1 to detect over-expressed TrkCT1 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-TrkCT1 to detect endogenous TrkCT1 in mouse cortex lysate (Lane 1). Lane 2 is TrkCT1 knock-out cortex lysate. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution. Personal Communication, V. Coppola, CCRNCI, Frederick, MD.
- Validation comment
- WB
