ANT-020-25UL

antibody from Invitrogen Antibodies

Targeting: NTRK3 TRKC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ANT-020-25UL

Provider

Invitrogen Antibodies

Product name

TrkC (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HEK-TrkC transfected cells (lanes 1 and 3) and HEK untransfected cells (lanes 2 and 4) lysates: - 1,2. Anti-TrkC (extracellular) Antibody (#ANT-020), (1:400).3,4. Anti-TrkC (extracellular) Antibody , preincubated with TrkC (extracellular) Blocking Peptide (#BLP-NT020).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HEK-TrkC transfected cells (lanes 1 and 3) and HEK untransfected cells (lanes 2 and 4) lysates: - 1,2. Anti-TrkC (extracellular) Antibody (#ANT-020), (1:400).3,4. Anti-TrkC (extracellular) Antibody , preincubated with TrkC (extracellular) Blocking Peptide (#BLP-NT020).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of TrkC in rat PC12 cells - Cell surface detection of TrkC in live intact ratpheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-TrkC (extracellular) Antibody (#ANT-020), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Nuclear staining using the cell permeable dye Hoechst 33342 (blue). C. Merge image of A and B.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of TrkC in rat PC12 cells - Cell surface detection of TrkC in live intact ratpheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-TrkC (extracellular) Antibody (#ANT-020), (1:50) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Nuclear staining using the cell permeable dye Hoechst 33342 (blue). C. Merge image of A and B.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of TrkC in rat dorsal root ganglia - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkC (extracellular) Antibody (#ANT-020), (1:100). A. TrkC labeling (red) appears in cell bodies. Note that the nerve fibers are not stained. B. Nuclear staining (blue) was visualized using Hoechst 33342. C. Merge of A and B.