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Learnsc-13039
antibody from Santa Cruz Biotechnology
Targeting: IKZF1
hIk-1, Hs.54452, IKAROS, LyF-1, PPP1R92, ZNFN1A1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-13039 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-13039, RRID:AB_2124699
- Product name
- Anti-IKZF1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references A cooperative microRNA-tumor suppressor gene network in acute T-cell lymphoblastic leukemia (T-ALL)
Cell-type-specific activation and repression of PU.1 by a complex of discrete, functionally specialized cis-regulatory elements.
Ikaros silences T-bet expression and interferon-gamma production during T helper 2 differentiation.
Promoter analysis of human glutamate carboxypeptidase II.
Notch3 and the Notch3-upregulated RNA-binding protein HuD regulate Ikaros alternative splicing.
Konstantinos J Mavrakis, Joni Van Der Meulen, Andrew L Wolfe, Xiaoping Liu, Evelien Mets, Tom Taghon, Aly A Khan, Manu Setty, Pieter Rondou, Peter Vandenberghe, Eric Delabesse, Yves Benoit, Nicholas B Socci, Christina S Leslie, Pieter Van Vlierberghe, Frank Speleman, Hans-Guido Wendel
Nature Genetics 2011 Jun;43(7):673-678
Nature Genetics 2011 Jun;43(7):673-678
Cell-type-specific activation and repression of PU.1 by a complex of discrete, functionally specialized cis-regulatory elements.
Zarnegar MA, Chen J, Rothenberg EV
Molecular and cellular biology 2010 Oct;30(20):4922-39
Molecular and cellular biology 2010 Oct;30(20):4922-39
Ikaros silences T-bet expression and interferon-gamma production during T helper 2 differentiation.
Thomas RM, Chen C, Chunder N, Ma L, Taylor J, Pearce EJ, Wells AD
The Journal of biological chemistry 2010 Jan 22;285(4):2545-53
The Journal of biological chemistry 2010 Jan 22;285(4):2545-53
Promoter analysis of human glutamate carboxypeptidase II.
Han L, Wong DL, Tsai G, Jiang Z, Coyle JT
Brain research 2007 Sep 19;1170:1-12
Brain research 2007 Sep 19;1170:1-12
Notch3 and the Notch3-upregulated RNA-binding protein HuD regulate Ikaros alternative splicing.
Bellavia D, Mecarozzi M, Campese AF, Grazioli P, Talora C, Frati L, Gulino A, Screpanti I
The EMBO journal 2007 Mar 21;26(6):1670-80
The EMBO journal 2007 Mar 21;26(6):1670-80
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 213, 118, 83, 50, 33, 26, 18
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 53
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human tonsil shows distinct nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
