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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX129438 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX129438, RRID:AB_2616071
- Product name
- Ikaros antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A kindred with mutant IKAROS and autoimmunity.
Resources for the Comprehensive Discovery of Functional RNA Elements.
Van Nieuwenhove E, Garcia-Perez JE, Helsen C, Rodriguez PD, van Schouwenburg PA, Dooley J, Schlenner S, van der Burg M, Verhoeyen E, Gijsbers R, Frietze S, Schjerven H, Meyts I, Claessens F, Humblet-Baron S, Wouters C, Liston A
The Journal of allergy and clinical immunology 2018 Aug;142(2):699-702.e12
The Journal of allergy and clinical immunology 2018 Aug;142(2):699-702.e12
Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IKZF1 antibody detects IKZF1 protein by western blot analysis.A. 50 ?g Jurkat whole cell lysate/extract B. 50 ?g Raji whole cell lysate/extractC. 50 ?g NCI-H929 whole cell lysate/extract 7.5% SDS-PAGEIKZF1 antibody (GTX129438) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IKZF1 antibody immunoprecipitates IKZF1 protein in IP experiments.IP samples: Raji whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of IKZF1 protein by 4 £gg IKZF1 antibody (GTX129438)7.5 % SDS-PAGEThe immunoprecipitated IKZF1 protein was detected by IKZF1 antibody (GTX129438) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with Jurkat chromatin extract and 5 £gg of either control rabbit IgG or anti-IKZF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.
