Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS1958 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- NF YA (K290) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 260-310 of Human NF-YA.
- Description
- The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold ¡ùhandshake¡ì motif in a manner analogous to the histone proteins, H2B and H2A, respectively.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of NF-YA (K290) pAb at 1:500 dilutionLane1:A549 whole cell lysateLane2:Raw264.7 whole cell lysate