TA319426

antibody from OriGene

Targeting: NFYA CBF-B, HAP2, NF-YA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319426

Provider

OriGene

Product name

Rabbit polyclonal anti-NF-Y antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-NF-Y antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

NFYA

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB of Rabbit Anti-NF-Y(A subunit) Antibody. Lane 1: CHO-7 cells in the absence (I) of cholesterol. Lane 2: CHO-7 cells in the presence (S) of cholesterol. Load: Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein per lane. Primary antibody: anti-NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

Validation comment

WB