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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- GTX48667 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48667, RRID:AB_11177833
- Product name
- NFYA antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 µg of total protein were subjected to analysis by SDS-PAGE and western blotting. After transfer, the membrane was probed using anti-NF-YA as the primary antibody at a 1:1,000 dilution, followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L]. Signal was developed using an ECL kit followed by autoradiography using a 30" exposure. The antibody recognizes NF-YA as a doublet of ~ 42 kDa in size.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Load: 35 ?g per lane. Primary antibody: NF-YA antibody at 1:1,000 for overnight at 4¢XC. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GeneTex's anti-NF-Y (A Subunit) antibody was diluted at 10 ?g/mL for 1 h at RT to detect NF-YA in human prostate tissue. Tissue was formalin fixed paraffin embedded. Antigen retrieval: not required. Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. The image shows the localization of antibody as the precipitated red signal with a hematoxylin purple nuclear counterstain. Localization: NF-Y is nuclear.
