Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-C18769 - Provider product page
- Provider
- LSBio
- Product name
- NFYA Antibody (N-Terminus) LS-C18769
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -70°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-NF-Y(A subunit) Antibody - Western Blot. CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 ug of total protein were subjected to analysis by SDS-PAGE and western blot. After transfer, the membrane was probed using anti-NF-YA as the primary antibody at a 1:1000 dilution, followed by reaction with HRP conjugated Goat-anti-Rabbit IgG [H&L]. Signal was developed using an ECL kit followed by autoradiography using a 30 exposure. The antibody recognizes NF-YA as a doublet of ~42 kD in size.