ABIN116535

antibody from antibodies-online

Targeting: NFYA CBF-B, HAP2, NF-YA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Gel shift (Recommended by provider)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

ABIN116535

Provider

antibodies-online

Product name

anti-Nuclear Transcription Factor Y, alpha (NFYA) (N-Term) antibody

Antibody type

Polyclonal

Antigen

NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Description

This product was prepared from monospecific antiserum by delipidation and defibrination.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

N-Term

Vial size

0.1 mL

Concentration

90.0 mg/mL (by Refractometry)

Storage

Store original vial at 2-8°C. After reconstitution store the antibody undiluted (in aliquots) at -20°C.

Handling

Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Figure 1. CHO-7 cells were cultured in the absence (I) or presence (S) of cholesterol. Equivalent aliquots of chromatin from each sample containing ~50 μg of total protein were subjected to analysis by SDSPAGE