Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS4864 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PLC ÉÇ3 (phospho S537) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human PLC ÉÇ3 around the phosphorylation site of Serine 537.
- Description
- A total of eight mammalian PLC isozymes have been described (PLC ÉÇ1, PLC ÉÇ2, PLC ÉÇ3, PLC ÉÇ4, PLC ÎÛ1, PLC ÎÛ2, PLC Ñ´1 and PLC Ñ´2) with molecular weights ranging from 85 to 150 kDa. The ÎÛ-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two ÎÛ-type enzymes, but not the ÉÇ and Ñ´ isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC ÉÇ1, PLC ÉÇ2 and PLC ÉÇ3 is mediated by the Ï« subunits of the Gq class of heterotrimeric G proteins and by certain ÉÇÎÛ G protein subunits. The regulatory mechanisms for PLC Ñ´1 and PLC Ñ´2 are as yet not resolved.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-PLC ¦Â3 (S537) pAb at 1:500 dilutionLane1:MCF-7 cell lysate treated with UVLane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate